3d computer aided design cad software  (Autodesk Inc)

Journal: Bioengineering

Article Title: Mesenchymal Stem Cell and Hematopoietic Stem and Progenitor Cell Co-Culture in a Bone-Marrow-on-a-Chip Device toward the Generation and Maintenance of the Hematopoietic Niche

doi: 10.3390/bioengineering11080748

Figure Lengend Snippet: Schematic representation of the passively perfused bone-marrow-on-a-chip (BMoC) device. ( a ) Exploded 3D illustration of the chip, consisting of two PDMS layers, the upper cell culture microchamber and the lower medium reservoir, separated by a porous membrane. The device is sealed on the top with a glass slide for cell observation. In magnification, a cross-section of the chip is presented, showing the mesenchymal stem cell (MSC) and hematopoietic stem and progenitor cell (HSPC) co-culture in the microchamber as well as the inserted interconnections reaching the microchamber. ( b ) Three-dimensional representation of the chip immersed in a 35 mm culture dish filled with medium. Passive perfusion of the chip is ensured via diffusion from the dish to the cell microchamber through the porous membrane.

Article Snippet: For the microchamber, 3D computer-aided design (CAD) software (Autodesk Fusion v. 2.0.18950) was used to design its mold, which was later realized in an aluminum substrate with Computer Numerical Control (CNC) micromachining.

Techniques: Cell Culture, Membrane, Co-Culture Assay, Diffusion-based Assay

Journal: Bioengineering

Article Title: Mesenchymal Stem Cell and Hematopoietic Stem and Progenitor Cell Co-Culture in a Bone-Marrow-on-a-Chip Device toward the Generation and Maintenance of the Hematopoietic Niche

doi: 10.3390/bioengineering11080748

Figure Lengend Snippet: The passively perfused BMoC device. ( a ) Photo of the device submerged in a 35 mm culture dish during the experimental process. Diametrically opposed channels were opened to allow for the medium’s replenishment in the chip reservoir. ( b ) SEM image (50× magnification) of a cross-section of the constructed BMoC device demonstrating its PDMS constituents, the cell culture microchamber, the medium reservoir, and the intervening PDMS membrane.

Article Snippet: For the microchamber, 3D computer-aided design (CAD) software (Autodesk Fusion v. 2.0.18950) was used to design its mold, which was later realized in an aluminum substrate with Computer Numerical Control (CNC) micromachining.

Techniques: Construct, Cell Culture, Membrane